Plasma Functionalized Carbon Interfaces for Biosensor Application: Toward the Real-Time Detection of Escherichia coli O157:H7

Gangwar, Rahul and Ray, Debjyoti and Rao, Karri Trinadha and Khatun, Sajmina and Subrahmanyam, Challapalli and Rengan, Aravind Kumar and Vanjari, Siva Rama Krishna (2022) Plasma Functionalized Carbon Interfaces for Biosensor Application: Toward the Real-Time Detection of Escherichia coli O157:H7. ACS Omega, 7 (24). pp. 21025-21034. ISSN 2470-1343

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Nonthermal plasma, a nondestructive, fast, and highly reproducible surface functionalization technique, was used to introduce desired functional groups onto the surface of carbon powder. The primary benefit is that it is highly scalable, with a high throughput, making it easily adaptable to bulk production. The plasma functionalized carbon powder was later used to create highly specific and low-cost electrochemical biosensors. The functional groups on the carbon surface were confirmed using NH3-temperature-programmed desorption (TPD) and X-ray photoelectron spectroscopy (XPS) analysis. In addition, for biosensing applications, a novel, cost-effective, robust, and scalable electrochemical sensor platform comprising in-house-fabricated carbon paste electrodes and a miniaturized E-cell was developed. Biotin-Streptavidin was chosen as a model ligand-analyte combination to demonstrate its applicability toward biosensor application, and then, the specific identification of the target Escherchia coli O157:H7 was accomplished using an anti-E. coli O157:H7 antibody-modified electrode. The proposed biosensing platform detected E. coli O157:H7 in a broad linear range of (1 × 10-1-1 × 106) CFU/mL, with a limit of detection (LOD) of 0.1 CFU/mL. In addition, the developed plasma functionalized carbon paste electrodes demonstrated high specificity for the target E. coli O157:H7 spiked in pond water, making them ideal for real-time bacterial detection. ©

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IITH Creators:
IITH CreatorsORCiD
Subrahmanyam, ChallapalliUNSPECIFIED
Rengan, Aravind Kumar
Vanjari, Siva Rama Krishna
Item Type: Article
Additional Information: The authors acknowledge the DST (Project No. DST/NM/NT/2021/01-1C), Ministry of Science & Technology, India, for funding the research.
Uncontrolled Keywords: Nonthermal plasma,highly reproducible surface functionalization technique, plasma functionalized carbon powder, highly specific and low-cost electrochemical biosensors
Subjects: Electrical Engineering
Biomedical Engineering
Divisions: Department of Biomedical Engineering
Department of Chemistry
Department of Electrical Engineering
Depositing User: . LibTrainee 2021
Date Deposited: 18 Jul 2022 11:55
Last Modified: 18 Jul 2022 11:55
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