Resonance assignment and secondary structure of the tandem harmonin homology domains of human RTEL1

Kumar, Niranjan and Ghosh, Meenakshi and Deepa, Akula and et al, . (2022) Resonance assignment and secondary structure of the tandem harmonin homology domains of human RTEL1. Biomolecular NMR Assignments, 16 (1). pp. 159-164. ISSN 1874-2718

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Regulator of telomere elongation helicase 1 (RTEL1) is an Fe-S cluster containing DNA helicase that plays important roles in telomere DNA maintenance, DNA repair, and genomic stability. It is a modular protein comprising an N-terminal helicase domain, two tandem harmonin homology domains 1 & 2 (HHD1 and HHD2), and a C-terminal C4C4 type RING domain. The N-terminal helicase domain disassembles the telomere t/D-loop and unwinds the G-quadruplex via its helicase activity. The C-terminal RING domain interacts with telomere DNA binding protein TRF2 and helps RTEL1 recruitment to the telomere. The tandem HHD1 and HHD2 are characterized as a putative protein-protein interaction domain and have recently been shown to interact with a DNA repair protein SLX4. Several mutations associated with Hoyeraal-Hreidarsson syndrome and pulmonary fibrosis have been found in HHD1 and HHD2 of RTEL1. However, these domains have not been characterized for their structures. We have expressed and purified HHD1 and HHD2 of human RTEL1 for their characterization using solution NMR spectroscopy. Here, we report near complete backbone and sidechain 1H, 13C and 15N chemical shift assignments and secondary structure of the HHD1 and HHD2 domains of human RTEL1. © 2022, The Author(s), under exclusive licence to Springer Nature B.V.

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Item Type: Article
Additional Information: This work is supported by an extramural research grant from the Department of Biotechnology (DBT), India [BT/PR15829/BRB/10/1469/2015].
Uncontrolled Keywords: Harmonin homology domain; Hoyeraal–Hreidarsson syndrome; NMR spectroscopy; Protein-protein interaction; RTEL1
Subjects: Others > Biological sciences
Others > Cellular biology
Biomedical Engineering
Divisions: Department of Biomedical Engineering
Depositing User: . LibTrainee 2021
Date Deposited: 27 Jul 2022 04:59
Last Modified: 27 Jul 2022 04:59
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