2-D material enhanced ultrasensitive electrochemical sensing of Pro-BNP peptide towards the risk-assessment of human heart

Goswami, Partha Pratim and Rotake, Dinesh Ramkrishna and Singh, Shiv Govind (2022) 2-D material enhanced ultrasensitive electrochemical sensing of Pro-BNP peptide towards the risk-assessment of human heart. Sensors and Actuators, B: Chemical, 357. pp. 1-10. ISSN 0925-4005

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The high morbidity, mortality, and huge numbers of affected individuals have delineated cardiovascular diseases as one of the most vulnerable global health burdens. Amongst the top proteins to reflect ventricular dysfunction, Brain-type natriuretic peptide (BNP or ProBNP) carries substantial potential towards estimating human heart risk, both at the prognostic and diagnostic level. Herein, clinically significant concentrations of ProBNP ranging from 100 fg/mL to 100 ng/mL are assessed via a hydrothermally synthesized 2-D SnS2 nanomaterial enhanced ultrasensitive and selective portable electrochemical biosensor. X-ray Diffraction, Scanning Electron Microscopy, Transmission Electron Microscopy, and Fourier Transform Infra-Red Spectroscopy are performed for material characterization. The transduction mechanism is realized through the covalent immobilization of the target-specific antibodies on the bioelectrode surface. Multiplex trigger signals, i.e., cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS), are employed to explore the antigen-antibody binding event. The sensitivity of the proposed sensor is found to be 11.7355 (ΔR/R) (ng/mL)−1cm−2 in case of EIS and 103.2396 (ΔIp/Ip) % (ng/mL)−1cm−2 in case of DPV with the lowest limit of detection being 0.225 fg/mL (SNR=3.3) for 5 mg/mL SnS2-DMF dispersion. Further, it is subjected to interference, stability, selectivity, and recovery studies in PBS and plasma matrix aiming at the on-field diagnostic performance. © 2022

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IITH Creators:
IITH CreatorsORCiD
Singh, Shiv Govindhttp://orcid.org/0000-0001-7319-879X
Item Type: Article
Additional Information: We acknowledge the Ministry of Education (MoE), Govt. of India, and the Department of Science and Technology (Financial assistance for the Cluster proposals under the Internet of Things (IoT) Research of Interdisciplinary Cyber Physical Systems (ICPS) Programme) for funding this research work. We thank Mr. Muriki Laxminarayana for performing TEM imaging and Mr. Rahul Gangwar and Mr. Madduri Suresh for performing SEM imaging for the research work.
Uncontrolled Keywords: Cardiovascular disease; Electrochemical sensors; Hydrothermal synthesis; Nanoparticles; ProBNP
Subjects: Electrical Engineering
Divisions: Department of Electrical Engineering
Depositing User: . LibTrainee 2021
Date Deposited: 02 Jul 2022 05:59
Last Modified: 13 Jul 2022 10:24
URI: http://raiith.iith.ac.in/id/eprint/9461
Publisher URL: http://doi.org/10.1016/j.snb.2022.131382
OA policy: https://v2.sherpa.ac.uk/id/publication/16792
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