Gut-specific arylphorin mediates midgut regenerative response against Cry-induced damage in Achaea janata

Dhania, N.K. and Chauhan, V.K. and Abhilash, D. and Thakur, V. and Chaitanya, R.K. and Dutta-Gupta, S. and Dutta-Gupta, A. (2021) Gut-specific arylphorin mediates midgut regenerative response against Cry-induced damage in Achaea janata. Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology, 255 (110600). ISSN 10964959

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Development of insect resistance to biopesticides is a current and pertinent global issue. Earlier, it was established that lepidopteran larvae can recover from Bt intoxication via a midgut regenerative response and subsequently generate resistance. Molecular aspects of restoration of the midgut integrity following toxin exposure are emerging recently. In the present study, we bring out the pivotal role of gut arylphorin in mediating the midgut regenerative response following sublethal Bt exposure in Achaea janata. Bt-induced midgut damage was characterized by microscopic analysis using differential interference contrast (DIC) and immunofluorescence (IF). Extensive disruption of brush-border membrane, associated with the underlying cytoskeletal alterations including F-actin, α-actin and β-tubulin was observed. Single-photon fluorescence microscopy combined with fluorescence lifetime imaging (FLIM) established the metabolic state associated with enhanced stem cell proliferation and migration from the basal side towards the luminal side following the damage. In-silico analysis revealed the phylogenetic relationship of gut arylphorin with closely related insect species and indicated the presence of two different subunits. Transient RNAi knockdown of the arylphorin resulted in diminished expression of mitotic Cyclin B mRNA levels. Human monoclonal Cyclin B antibody cross-reactivity with the Cyclin B located in the stem cells further validate the role of arylphorin as the mitogenic factor responsible for stem cell proliferation and epithelial regeneration. An in-depth understanding of resistance mechanisms will aid in the design of new strategies for the long-term usage and efficacy of Bt technology against pest control. © 2021

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IITH Creators:
IITH CreatorsORCiD
Gupta, Shourya DuttaUNSPECIFIED
Item Type: Article
Uncontrolled Keywords: Arylphorins, Bacillus thuringiensis (Bt), Cyclin B, Gut stem cells, Midgut regeneration, Pest resistance
Subjects: Others > Metallurgy Metallurgical Engineering
Materials Engineering > Materials engineering
Divisions: Department of Material Science Engineering
Depositing User: Mrs Haseena VKKM
Date Deposited: 25 Apr 2022 05:09
Last Modified: 25 Apr 2022 05:09
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