Cell encapsulated and microenvironment modulating microbeads containing alginate hydrogel system for bone tissue engineering

Veernala, Induvahi and Roopmani, Purandhi and Singh, Ruby and Hasan, Uzma and Giri, Jyotsnendu (2021) Cell encapsulated and microenvironment modulating microbeads containing alginate hydrogel system for bone tissue engineering. Progress in Biomaterials, 10 (2). pp. 131-150. ISSN 2194-0509

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Functional tissue regeneration using synthetic biomaterials requires proliferation and heterotypic differentiation of stem/progenitor cells within a specialized heterogeneous (biophysical-biochemical) microenvironment. The current techniques have limitations to develop synthetic hydrogels, mimicking native extracellular matrix porosity along with heterogeneous microenvironmental cues of matrix mechanics, degradability, microstructure and cell-cell interactions. Here, we have developed a microenvironment modulating system to fabricate in situ porous hydrogel matrix with two or more distinct tailored microenvironmental niches within microbeads and the hydrogel matrix for multicellular tissue regeneration. Electrosprayed pectin-gelatin blended microbeads and crosslinked alginate hydrogel system help to tailor microenvironmental niches of encapsulated cells where two different cells are surrounded by a specific microenvironment. The effect of different microenvironmental parameters associated with the microbead/hydrogel matrix was evaluated using human umbilical-cord mesenchymal stem cells (hUCMSCs). The osteogenic differentiation of hUCMSCs in the hydrogel matrix was evaluated for bone tissue regeneration. This will be the first report on microenvironment modulating microbead-hydrogel system to encapsulate two/more types of cells in a hydrogel, where each cell is surrounded with distinct niches for heterogeneous tissue regeneration.

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IITH Creators:
IITH CreatorsORCiD
Giri, JyotsnenduUNSPECIFIED
Item Type: Article
Uncontrolled Keywords: Electrospraying; Microenvironment modulating hydrogel; Microenvironment modulating microbead; Stem cells; Tissue engineering.
Subjects: Biomedical Engineering
Divisions: Department of Biomedical Engineering
Depositing User: . LibTrainee 2021
Date Deposited: 10 Jul 2021 09:30
Last Modified: 10 Jul 2021 09:30
URI: http://raiith.iith.ac.in/id/eprint/8220
Publisher URL: http://doi.org/10.1007/s40204-021-00158-3
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