Nanometric depth phase imaging using low-cost on-chip lensless inline holographic microscopy

Gurram, Hanu Phani Ram and Galande, Ashwini S. and John, Renu (2020) Nanometric depth phase imaging using low-cost on-chip lensless inline holographic microscopy. Optical Engineering, 59 (104105). ISSN 0091-3286

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Lensless microscopes are simple, portable, and cost effective compared with the sophisticated microscopes of today that require high-end objectives, lenses, and filters. We demonstrate a lensless on-chip phase microscope based on the holographic principle to image 3D nanometric depth information from transparent and weakly scattering biological samples. We characterize the microscope using standard quantitative phase resolution target (PRT) charts with feature depths from 50 to 350 nm and report a signal-to-noise ratio value of 23 dB. Further, we apply a gradient descent-based constrained optimization approach for phase retrieval to eliminate the twin image and noise from the hologram. The device, operating as an inline holographic microscope, can perform quantitative phase imaging with nanometric depth sensitivity for a field of view of 29.4 mm2 using an LED-based light source butt-coupled to an optical fiber cable.

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IITH Creators:
IITH CreatorsORCiD
John, Renu
Item Type: Article
Uncontrolled Keywords: Biological samples; Depth information; Depth sensitivity; Gradient descent; Lensless in-line holographic microscopies; Phase resolution; Phase retrieval; Quantitative phase imaging;Constrained optimization; Cost effectiveness; Costs; Gradient methods; Holograms; Light sources; Microscopes; Optical fibers
Subjects: Materials Engineering > Materials engineering
Materials Engineering > Nanostructured materials, porous materials
Divisions: Department of Biomedical Engineering
Depositing User: . LibTrainee 2021
Date Deposited: 25 Jun 2021 06:22
Last Modified: 25 Jun 2021 06:22
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