Cell Boundary Confinement Sets the Size and Position of the E. coli Chromosome

Wu, Fabai and Swain, Pinaki and Kuijpers, L and et al, . (2019) Cell Boundary Confinement Sets the Size and Position of the E. coli Chromosome. Current Biology, 29 (13). 2131-2144.e4. ISSN 09609822

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Although the spatiotemporal structure of the genome is crucial to its biological function, many basic questions remain unanswered on the morphology and segregation of chromosomes. Here, we experimentally show in Escherichia coli that spatial confinement plays a dominant role in determining both the chromosome size and position. In non-dividing cells with lengths increased to 10 times normal, single chromosomes are observed to expand > 4-fold in size. Chromosomes show pronounced internal dynamics but exhibit a robust positioning where single nucleoids reside robustly at mid-cell, whereas two nucleoids self-organize at 1/4 and 3/4 positions. The cell-size-dependent expansion of the nucleoid is only modestly influenced by deletions of nucleoid-associated proteins, whereas osmotic manipulation experiments reveal a prominent role of molecular crowding. Molecular dynamics simulations with model chromosomes and crowders recapitulate the observed phenomena and highlight the role of entropic effects caused by confinement and molecular crowding in the spatial organization of the chromosome. Imaging chromosomes in E. coli within a broad length range, Wu et al. observe that chromosome size and position strongly depend on cell size. They provide evidence that this arises from a confinement-modulated entropic repulsion between chromosome and cytosolic crowders, highlighting the importance of confinement effects in cellular organization.

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IITH Creators:
IITH CreatorsORCiD
Item Type: Article
Uncontrolled Keywords: bacterial nucleoid, cell boundary confinement, chromosome segregation, chromosome size, crowders,Indexed in Scopus
Subjects: Chemistry
Divisions: Department of Chemistry
Depositing User: Library Staff
Date Deposited: 10 Oct 2019 11:23
Last Modified: 10 Oct 2019 11:23
URI: http://raiith.iith.ac.in/id/eprint/6530
Publisher URL: http://doi.org/10.1016/j.cub.2019.05.015
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