DNA bending facilitates the error-free DNA damage tolerance pathway and upholds genome integrity

V, gonzalez-huici and B, szakal and Eerappa, Rajakumara and et al, . (2014) DNA bending facilitates the error-free DNA damage tolerance pathway and upholds genome integrity. The EMBO Journal.

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DNA replication is sensitive to damage in the template. To bypass lesions and complete replication, cells activate recombination-mediated (error-free) and translesion synthesis-mediated (error-prone) DNA damage tolerance pathways. Crucial for error-free DNA damage tolerance is template switching, which depends on the formation and resolution of damage-bypass intermediates consisting of sister chromatid junctions. Here we show that a chromatin architectural pathway involving the high mobility group box protein Hmo1 channels replication-associated lesions into the error-free DNA damage tolerance pathway mediated by Rad5 and PCNA polyubiquitylation, while preventing mutagenic bypass and toxic recombination. In the process of template switching, Hmo1 also promotes sister chromatid junction formation predominantly during replication. Its C-terminal tail, implicated in chromatin bending, facilitates the formation of catenations/hemicatenations and mediates the roles of Hmo1 in DNA damage tolerance pathway choice and sister chromatid junction formation. Together, the results suggest that replication-associated topological changes involving the molecular DNA bender, Hmo1, set the stage for dedicated repair reactions that limit errors during replication and impact on genome stability.

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IITH Creators:
IITH CreatorsORCiD
Eerappa, Rajakumarahttp://orcid.org/0000-0002-9341-0070
Item Type: Article
Subjects: Others > Biotechnology
Divisions: Department of Biotechnology
Depositing User: Library Staff
Date Deposited: 07 Aug 2019 12:01
Last Modified: 19 Aug 2019 04:43
URI: http://raiith.iith.ac.in/id/eprint/5887
Publisher URL: doi: 10.1002/embj.201387425
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