Effects of Sodium Benzoate Using Zebrafish Animal Model

P, Srinithi and Bhargava, Anamika (2018) Effects of Sodium Benzoate Using Zebrafish Animal Model. Masters thesis, Indian Institute of Technology Hyderabad.

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The advent of food preservation has solved many food borne diseases and it also increased the food security over the years around the globe. From traditional boiling to modern day artificial food additives, there are numerous ways of preserving food items. The one that concerns most of the people around the globe is the addition of chemicals as preservatives in food items like jams, pickles, beverages etc. The most used chemical preservative sodium benzoate (SB) has gained more lime light due to some of its controversial effects on human health. Our study aims to investigate the toxic effects of sodium benzoate on the development, behaviour and oxidative stress in zebrafish embryos. We exposed zebrafish larvae of 5 hpf with 100 to 2000 ppm of SB and studied developmental, behavioural and gene expression changes. Our studies indicated that SB induced morphological abnormalities like pericardial edema, Yolk sac edema and tail bending. The malformations were more pronounced with the increase in dose of SB and time of exposure and also SB was found to delay the hatching process. The LC50 was found to be around 400 ppm at 48 hours of drug exposure. The behavioural experiment indicated increased thigmotaxis in treated larvae and there was a two fold increase in the gene expression of (glutathione reductase) and no change in (glyoxalase1) expression Our study strongly supports the toxic effects on vertebrates at increasing doses. Thus, we suggest caution in the extensive use of this preservative in processed and convenience foods.

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IITH Creators:
IITH CreatorsORCiD
Bhargava, Anamikahttp://orcid.org/0000-0002-1048-3427
Item Type: Thesis (Masters)
Uncontrolled Keywords: Sodium Benzoate, Mortality, Hatching Anxiety Gas
Subjects: Others > Biotechnology
Divisions: Department of Biotechnology
Depositing User: Team Library
Date Deposited: 29 Jun 2018 10:08
Last Modified: 29 Jun 2018 10:08
URI: http://raiith.iith.ac.in/id/eprint/4102
Publisher URL:
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