Digital Microfluidic (DMF) devices based on electrowetting on dielectric (EWOD) for biological applications

Kshirasagar, Saniya Sanjay and Unni, H N (2018) Digital Microfluidic (DMF) devices based on electrowetting on dielectric (EWOD) for biological applications. Masters thesis, Indian Institute of Technology, Hyderabad.

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Microfluidic devices have been used in various applications including automated analysis systems, biological applications like DNA sequencing, antigen-antibody reactions, protein studies, chemical applications, single cell studies, etc. Microfluidic devices are primarily categorised into two types. First are continuous microfluidic devices. These devices consist of predefined microchannels, micro-valves, and syringe pumps. Fluid is continuously flowing in these channels. The second type is digital microfluidic platforms. In this type, MXN array of electrodes is patterned on non-conducting substrate. Fluid is discretized to form tiny droplets. These droplets are transported, mixed and split using external electric field. Digital microfluidic devices are configurable as there are no permanently etched channels. Also, they have high throughput. Multiple reactions can be performed on the same platform at the same time. The time taken to complete one reaction is less compared to the continuous devices. Thus they help in faster analysis. These devices are controlled by electrical field and thus unlike continuous devices, digital microfluidic devices are free from mechanically moving parts. Digital microfluidic devices may suffer from charge accumulation due to electrostatic forces. Also, voltage levels applied play an important role. The applied voltage has to be enough to move droplets but should not cause electrolysis of the liquid used. Also voltage switching time between electrodes and frequency applied are important. These parameters can change the mixing quality. In this work, 2D simulations of droplet manipulation due to voltage application, transport and mixing are carried out. Also digital microfluidic device is designed and fabricated to carry out biological mixing experiments.

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IITH Creators:
IITH CreatorsORCiD
Unni, Harikrishnan NarayananUNSPECIFIED
Item Type: Thesis (Masters)
Uncontrolled Keywords: DMF, EWOD< Electrwetting
Subjects: Biomedical Engineering
Divisions: Department of Biomedical Engineering
Depositing User: Team Library
Date Deposited: 18 Jun 2018 05:05
Last Modified: 07 Mar 2022 09:12
Publisher URL:
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