Centrifugal microfluidics for sorting immune cells from whole blood

Yu, Z T F and Joseph, J G and Liu, S X and Cheung, M K and Haffey, P J and Kurabayashi, K and Fu, J (2017) Centrifugal microfluidics for sorting immune cells from whole blood. Sensors and Actuators, B: Chemical, 245. pp. 1050-1061. ISSN 0925-4005

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Sorting and enumeration of immune cells from blood are critical operations involved in many clinical applications. Conventional methods for sorting and counting immune cells from blood, such as flow cytometry and hemocytometers, are tedious, inaccurate, and difficult for implementation for point-of-care (POC) testing. Herein we developed a microscale centrifugal technology termed Centrifugal Microfluidic Chip (CMC) capable of sorting immune cells from blood and in situ cellular analysis in a laboratory setting. Operation of the CMC entailed a blood specimen layered on a density gradient medium and centrifuged in microfluidic channels where immune cell subpopulations could rapidly be sorted into distinct layers according to their density differentials. We systematically studied effects of different blocking molecules for surface passivation of the CMC. We further demonstrated the applicability of CMCs for rapid separation of minimally processed human whole blood without affecting immune cell viability. Multi-color imaging and analysis of immune cell distributions and enrichment such as recovery and purity rates of peripheral blood mononuclear cells (PBMCs) were demonstrated using CMCs. Given its design and operation simplicity, portability, blood cell sorting efficiency, and in situ cellular analysis capability, the CMC holds promise for blood-based diagnosis and disease monitoring in POC applications.

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IITH Creators:
IITH CreatorsORCiD
Item Type: Article
Uncontrolled Keywords: Centrifugation; Microfluidics; Blood cell sorting; Immune cells
Subjects: Chemistry > Electrochemistry
Chemistry > Analytical chemistry
Divisions: Department of Chemical Engineering
Depositing User: Team Library
Date Deposited: 23 Jun 2017 04:46
Last Modified: 23 Jun 2017 04:46
URI: http://raiith.iith.ac.in/id/eprint/3283
Publisher URL: https://doi.org/10.1016/j.snb.2017.01.113
OA policy: http://www.sherpa.ac.uk/romeo/issn/0925-4005/
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