EK3D: an E. coli K antigen 3-dimensional structure database

Kunduru, B R and Nair, S A and Rathinavelan, Thenmalarchelvi (2015) EK3D: an E. coli K antigen 3-dimensional structure database. Nucleic Acids Research. pp. 1-7. ISSN 0305-1048

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A very high rate of multidrug resistance (MDR) seen among Gram-negative bacteria such as Escherichia, Klebsiella, Salmonella, Shigella, etc. is a major threat to public health and safety. One of the major virulent determinants of Gram-negative bacteria is capsular polysaccharide or K antigen located on the bacterial outer membrane surface, which is a potential drug & vaccine target. It plays a key role in host–pathogen interactions as well as host immune evasion and thus, mandates detailed structural information. Nonetheless, acquiring structural information of K antigens is not straightforward due to their innate enormous conformational flexibility. Here, we have developed a manually curated database of K antigens corresponding to various E. coli serotypes, which differ from each other in their monosaccharide composition, linkage between the monosaccharides and their stereoisomeric forms. Subsequently, we have modeled their 3D structures and developed an organized repository, namely EK3D that can be accessed through www.iith.ac.in/EK3D/. Such a database would facilitate the development of antibacterial drugs to combat E. coli infections as it has evolved resistance against 2 major drugs namely, third-generation cephalosporins and fluoroquinolones. EK3D also enables the generation of polymeric K antigens of varying lengths and thus, provides comprehensive information about E. coli K antigens.

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IITH Creators:
IITH CreatorsORCiD
Rathinavelan, Thenmalarchelvihttp://orcid.org/0000-0002-1142-0583
Item Type: Article
Additional Information: The authors thank high performance computing facility of IITH, CDAC and IUAC.
Subjects: Others > Biotechnology
Divisions: Department of Biotechnology
Depositing User: Team Library
Date Deposited: 01 Dec 2015 05:09
Last Modified: 26 Sep 2017 04:20
URI: http://raiith.iith.ac.in/id/eprint/2050
Publisher URL: https://doi.org/10.1093/nar/gkv1313
OA policy: http://www.sherpa.ac.uk/romeo/issn/0305-1048/
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