Smartphone-powered, ultrasensitive, and selective, portable and stable multi-analyte chemiresistive immunosensing platform with PPY/COOH-MWCNT as bioelectrical transducer: Towards point-of-care TBI diagnosis

Singh, Shiv Govind (2023) Smartphone-powered, ultrasensitive, and selective, portable and stable multi-analyte chemiresistive immunosensing platform with PPY/COOH-MWCNT as bioelectrical transducer: Towards point-of-care TBI diagnosis. Bioelectrochemistry, 151. p. 108391. ISSN 1567-5394

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Abstract

Traumatic Brain Injury, one of the significant causes of mortality and morbidity, affects worldwide and continues to be a diagnostic challenge. The most desirable and partially met clinical need is to simultaneously detect the disease-specific-biomarkers in a broad range of readily available body fluids on a single platform with a rapid, low-cost, ultrasensitive and selective device. Towards this, an array of interdigitated microelectrodes was fabricated on commercially existing low-cost single-side copper cladded printed-circuit-board substrate followed by the bioelectrodes preparation through covalent immobilization of brain injury specific biomarkers on carboxylic functionalized multi-walled carbon nanotubes embedded polypyrrole nanocomposite modified interdigitated microelectrodes. Subsequently, the immunological binding events were transduced as the normalized change in bioelectrode resistance with and without the target analyte via current-voltage analysis. As proof of concept, current-voltage responses were primarily recorded using a conventional probe station, and later, a portable handheld-electronic-readout was developed for the point-of-care application. The data compilation and analysis were carried out using the in-house developed android-based mobile app. Notably, the smartphone powered the readout through a PL-2303 serial connector, avoiding integrating power sources with the readout. Further, this technology can be adapted to other point-of-care biosensing applications.

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IITH Creators:
IITH CreatorsORCiD
Singh, Shiv Govindhttp://orcid.org/0000-0001-7319-879X
Item Type: Article
Uncontrolled Keywords: Array of interdigitated microelectrodes; Chemiresistive immunosensing; Point-of-care diagnosis; Pyrrole nanocomposite based bioelectrical transducer; Smartphone-powered handheld portable electronic readout; Traumatic brain injury; Nanocomposites; Article; comparative study; data aggregation; data analysis; electric current; electric potential; immobilization; limit of detection; matrix effect; measurement repeatability; point of care testing; proof of concept; reproducibility; synthesis; traumatic brain injury; genetic procedures; human; point of care system; smartphone; Array of interdigitated microelectrode; Chemiresistive immunosensing; Electronic readout; Handhelds; Immunosensing; Interdigitated microelectrodes; Point of care; Point-of-care diagnose; Portable electronics; Pyrrole nanocomposite based bioelectrical transducer; Smart phones; Smartphone-powered handhold portable electronic readout; Traumatic Brain Injuries; biological marker; carboxylic acid; copper; glial fibrillary acidic protein; multi walled nanotube; nanocomposite; polypyrrole; protein; ubiquitin carboxy terminal hydrolase l1 protein; unclassified drug; carbon nanotube; polymer; pyrrole derivative; Biomarkers; Brain; Costs; Diagnosis; Microelectrodes; Multiwalled carbon nanotubes (MWCN); Neurophysiology; Printed circuit boards; Smartphones; Transducers; Tumors; Biomarkers; Biosensing Techniques; Brain Injuries, Traumatic; Humans; Nanotubes, Carbon; Point-of-Care Systems; Polymers; Pyrroles; Smartphone
Subjects: Electrical Engineering
Electrical Engineering > Electrical and Electronic
Divisions: Department of Electrical Engineering
Depositing User: Mr Nigam Prasad Bisoyi
Date Deposited: 28 Nov 2023 04:08
Last Modified: 28 Nov 2023 04:08
URI: http://raiith.iith.ac.in/id/eprint/11757
Publisher URL: https://doi.org/10.1016/j.bioelechem.2023.108391
OA policy: https://v2.sherpa.ac.uk/id/publication/12808
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