FeS2-based aerogel as a flexible low-cost substrate for rapid SERS detection of histamine in biofluids

Badhulika, Sushmee (2023) FeS2-based aerogel as a flexible low-cost substrate for rapid SERS detection of histamine in biofluids. New Journal of Chemistry, 47 (24). pp. 11615-11622. ISSN 1144-0546

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Abstract

Histamine is an organic nitrogenous compound released from mast cells, often as part of an instant immune response. Life-threatening anaphylaxis can occur with significant histamine intolerance, which is also referred to as histaminosis caused by overproduction of histamine in the body. Herein, we demonstrate the synthesis of FeS2-incorporated rGO aerogel (FeS2-AG) by lyophilization, as an active SERS substrate for the detection of histamine in blood serum. The morphological characterization revealed the nanoflower structure of FeS2 and uniform porosity of the aerogel, in which the nanoflower structure of FeS2 was homogenously distributed on the surface of the porous aerogel. The cubic structure of FeS2 nanoflowers was revealed by X-ray diffraction and Fourier transform infrared spectroscopy demonstrated the characteristic peak at ∼1176 cm−1 which is related to pyrite surface chemistry of FeS2 nanoparticles. The as-fabricated sensor exhibited an enhancement factor of 1.61 × 107 with a limit of detection of 0.08 ng mL−1. The substrate exhibited a good selectivity and linear range of detection towards histamine in the presence of 2-fold concentration of ascorbic acid, glucose, urea, and uric acid. This excellent performance of the sensor can be ascribed to the chemical enhancement occurring via the interaction of oxy functional groups of aerogel and multiple coordination sites (ferrous ions (Fe2+)) of FeS2 with histamine molecules causing reconstruction of the band gap. This newly formed energy band further leads to the occurrence of the Raman resonance effect, providing an enhancement in the SERS signal over that of the Raman signal obtained from bulk histamine. The sensor showed good recovery percentage of 97.25% from real samples, highlighting the efficiency of the as-fabricated sensor towards histamine detection.

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IITH Creators:
IITH CreatorsORCiD
Badhulika, Sushmeeorcid.org/0000-0003-3237-3031
Item Type: Article
Uncontrolled Keywords: Ascorbic acid; Costs; Energy gap; Fourier transform infrared spectroscopy; Nanoflowers; Pyrites; Raman spectroscopy; Substrates; Surface chemistry; Urea; aerogel; ascorbic acid; ferrous sulfide; glucose; histamine; nanoflower; pyrite; urea; uric acid; Biofluids; Fabricated sensors; Histamines; Immune response; Low-costs; Mast cells; Nitrogenous compounds; Organics; SERS; SERS substrate; Article; calibration; chemical structure; Fourier transform infrared spectroscopy; freeze drying; limit of detection; morphological trait; physical chemistry; porosity; scanning electron microscopy; sensitivity analysis; serum; surface enhanced Raman spectroscopy; surface property; synthesis; X ray diffraction; Aerogels
Subjects: Electrical Engineering
Divisions: Department of Electrical Engineering
Depositing User: Mr Nigam Prasad Bisoyi
Date Deposited: 26 Nov 2023 05:37
Last Modified: 26 Nov 2023 05:37
URI: http://raiith.iith.ac.in/id/eprint/11755
Publisher URL: https://doi.org/10.1039/d3nj01736b
OA policy: https://v2.sherpa.ac.uk/id/publication/18027
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