Spiral microfluidics enhanced isolation of epithelial cells from infected mice urine

Duraiswamy, Suhanya and Yung, L.Y.L. and Chen, S.L. (2020) Spiral microfluidics enhanced isolation of epithelial cells from infected mice urine. In: 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020, 4-9 October 2020, Virtual, Online.

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Abstract

Spiral microfluidics has been used in this study to isolate infected epithelial cells containing intracellular bacterial communities (IBCs) from other cells (RBCs, WBCs, and bacteria), in urine. Epithelial cells in human urine are 20 μm to 160 μm in size and hence traditional size based spiral microfluidic isolation cannot be used effectively. We isolated ~60% of epithelial cells by combining Dean Flow Fractionation with switched focusing mode at \textgreater 98% purity. We then use the proposed method for the isolation of epithelial cells from infected mice urine and show the presence of IBCs in the isolated cells as a proof of concept. © 2020 CBMS-0001

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IITH Creators:
IITH CreatorsORCiD
Duraiswamy, Suhanyahttps://orcid.org/0000-0002-1803-5389
Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Intracellular Bacterial Communities (IBCs), Spiral microfluidics, Urine Tract Infections (UTI)
Subjects: Electrical Engineering
Divisions: Department of Chemical Engineering
Depositing User: . LibTrainee 2021
Date Deposited: 23 Nov 2022 07:27
Last Modified: 23 Nov 2022 07:27
URI: http://raiith.iith.ac.in/id/eprint/11336
Publisher URL:
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