Thickening of Ectatic Cornea through Regeneration Using Decellularized Corneal Matrix Injectable Hydrogel: A Strategic Advancement to Mitigate Corneal Ectasia

Chameettachal, Shibu and Puranik, Charuta J and Veluthedathu, Mohamed Nijas and Chalil, Najathulla Bhagavathi and John, Renu and Pati, Falguni (2021) Thickening of Ectatic Cornea through Regeneration Using Decellularized Corneal Matrix Injectable Hydrogel: A Strategic Advancement to Mitigate Corneal Ectasia. ACS Applied Bio Materials, 4 (9). pp. 7300-7313. ISSN 2576-6422

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Ectatic corneal diseases are a group of eye disorders characterized by progressive thinning and outward bulging of the cornea, resulting in vision impairment. A few attempts have been made to use cornea-derived extracellular matrix hydrogels for corneal tissue engineering; however, no studies have investigated its application in corneal ectasia. In this study, we have first developed an animal surgical model that mimics a few specific phenotypes of ectatic cornea. Later, we investigated the potential of decellularized cornea matrix hydrogels (dCMH) from both human and bovine sources in increasing the thickness of the cornea in the developed surgical model. Our data advocate that surgical stromal depletion can be followed to establish ectatic models and can also provide information on the biocompatibility of materials, its integration with native stroma, degradation over time, and tissue remodeling. We observed that dCMH from both sources could integrate with ectatic thin corneal stroma and helps in regaining the thickness by regenerating a reasonably functional and transparent stroma; however, no significant difference was spotted between the dCMH made from human and bovine corneal tissue sources. Hence, this study is a promising step toward developing a non-invasive technique for the treatment of corneal ectasia by using dCMH. © 2021 American Chemical Society.

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IITH Creators:
IITH CreatorsORCiD
John, Renu
Pati, Falguni
Item Type: Article
Additional Information: Authors would like to acknowledge financial support from the Society for Research and Initiatives for Sustainable Technologies and Institutions (SRISTI) and Biotechnology Industry Research Assistance Council, Govt. of India, for providing the BIRAC-SRISTI award grant. We thank Dr. Dhoolappa Melinamani, Veterinary College, Shivamogga, and Mr. Sreejith Paramadam for their support in finding resources and Frigerio Conserva Allana Private Limited, IDA, Algole Road, Zaheerabad, Sangareddy (Dist), Telangana 502220, for supplying the bovine corneas used in the study.
Uncontrolled Keywords: corneal ectasia; corneal regeneration; corneal thinning; decellularized cornea matrix hydrogel; stromal depletion
Subjects: Biomedical Engineering
Divisions: Department of Biomedical Engineering
Depositing User: . LibTrainee 2021
Date Deposited: 04 Oct 2022 13:14
Last Modified: 04 Oct 2022 13:14
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