Smooth muscle matrix bioink promotes myogenic differentiation of encapsulated adipose‐derived stem cells

Yeleswarapu, Sriya and Chameettachal, Shibu and Bera, Ashis Kumar and Pati, Falguni (2022) Smooth muscle matrix bioink promotes myogenic differentiation of encapsulated adipose‐derived stem cells. Journal of Biomedical Materials Research Part A. ISSN 1549-3296

Full text not available from this repository. (Request a copy)


Hydrogels derived from decellularized extracellular matrices (dECM) can mimic the biochemical composition of the native tissue. They can also act as a template to culture reseeded cells in vitro. However, detergent-based decellularization methods are known to alter the biochemical compositions, thereby compromising the bioactive potential of dECM. This study proposes a facile detergent-free method to achieve dECM from smooth muscle tissue. We have used the muscle layer of caprine esophageal tissue and decellularized using hypo and hyper-molar sodium chloride solutions alternatingly. Then, a hydrogel was prepared from this decellularized smooth muscle matrix (dSMM) and characterized thoroughly. A comparative analysis of the dSMM prepared with our protocol with the existing detergent-based protocol suggests successful and comparable decellularization with minimal residual DNA content. Interestingly, an 8.78-fold increase in sulfated glycosaminoglycans content and 1.62-fold increased collagen content indicated higher retention of ECM constituents with NaCl-based decellularization strategy. Moreover, the dSMM gel induces differentiation of the encapsulated adipose-derived mesenchymal stem cells toward smooth muscle cells (SMCs) as observed by their expression of alpha-smooth muscle actin and smooth muscle myosin heavy chain, the hallmarks of SMCs. Finally, we optimized the process parameter for productive bioprinting with this dSMM bioink and fabricated 3D muscle constructs. Our results suggest that dSMM has the potential to be used as a bioink to engineer personalized esophageal tissues. © 2022 Wiley Periodicals LLC.

[error in script]
IITH Creators:
IITH CreatorsORCiD
Pati, Falguni
Item Type: Article
Additional Information: The authors would like to acknowledge the financial support from Department of Science and Technology, Ministry of Science and Technology, Govt. of India under the FIST program (SR/FST/LSI‐683/2016(C)).
Uncontrolled Keywords: 3D bioprinting; decellularized smooth muscle matrix; esophagus; hypo/hyper NaCl; stem cell differentiation
Subjects: Biomedical Engineering
Divisions: Department of Biomedical Engineering
Depositing User: . LibTrainee 2021
Date Deposited: 16 Aug 2022 08:28
Last Modified: 16 Aug 2022 08:28
Publisher URL:
OA policy:
Related URLs:

Actions (login required)

View Item View Item
Statistics for RAIITH ePrint 10185 Statistics for this ePrint Item