Lensless holographic microscope of biological samples

Ram, G Hanu Phani and Bansode, Vaibhav and John, Renu (2018) Lensless holographic microscope of biological samples. In: Computational Optical Sensing and Imaging, 25–28 June 2018, Florida, United States.

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Abstract

Lensless holographic microscopy shows high potential to be a practical point of care diagnostic tool due to its unique features like compactness, simple prototype, portability and on-chip operation. In the present work, a deep violet (370nm, 10 nm FWHM) LED at low power is used as a low coherent light source for lensless 3-D holographic imaging of live cells and microparticles. An on-chip Fresnel hologram of the sample is recorded in on-axis geometry on a high resolution CMOS camera and 3-D phase reconstruction has been performed eliminating the twin images using an optimization approach.

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IITH Creators:
IITH CreatorsORCiD
John, Renuhttps://orcid.org/0000-0003-3254-2472
Item Type: Conference or Workshop Item (Paper)
Subjects: Biomedical Engineering
Divisions: Department of Biomedical Engineering
Depositing User: Team Library
Date Deposited: 29 Oct 2019 09:41
Last Modified: 29 Oct 2019 09:41
URI: http://raiith.iith.ac.in/id/eprint/6885
Publisher URL: http://doi.org/10.1364/3D.2018.JTu4A.36
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