Quantitative label-free technique for morphological evaluation of human sperm—a promising tool in semen evaluation

Poola, Praveen Kumar and Jayaraman, Varshini and John, Renu (2018) Quantitative label-free technique for morphological evaluation of human sperm—a promising tool in semen evaluation. OSA Continuum, 1 (4). p. 1215. ISSN 2578-7519

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Abstract

We present results from non-interferometric quantitative phase imaging (QPI) for thickness estimation and morphological analysis of human spermatozoa. QPI along with algorithms for image processing enable localization and quantification of subcellular structures of spermatozoa, including the acrosomal, nuclear, mid-piece, and tail regions. This approach based on the transport of intensity equation (TIE) using a simple brightfield microscope renders quantitative phase image with nanometric depth sensitivity, allowing the precise subcellular structural resolution of human spermatozoa. We demonstrate the potential of TIE in real-time phase imaging and the morphological analysis of spermatozoa. We first calibrate the TIE using microbeads and a standard phase target of known dimensions. Image processing for quantitative volume estimation of nuclei and acrosomal regions is carried out on the reconstructed quantitative phase images. We also apply a Hilbert transform-based image processing algorithm to the retrieved phase for visualization of vacuoles present on the sperm head.

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IITH Creators:
IITH CreatorsORCiD
John, Renuhttps://orcid.org/0000-0003-3254-2472
Item Type: Article
Subjects: Biomedical Engineering
Divisions: Department of Biomedical Engineering
Depositing User: Team Library
Date Deposited: 29 Oct 2019 09:33
Last Modified: 29 Oct 2019 09:33
URI: http://raiith.iith.ac.in/id/eprint/6884
Publisher URL: http://doi.org/10.1364/OSAC.1.001215
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