Cardiac BIN1 folds T-tubule membrane, controlling ion flux and limiting arrhythmia

Hong, TingTing and Yang, Huanghe and Bhargava, Anamika and et al, . (2014) Cardiac BIN1 folds T-tubule membrane, controlling ion flux and limiting arrhythmia. Nature Medicine, 20 (6). pp. 624-632. ISSN 1078-8956

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Abstract

Cardiomyocyte T tubules are important for regulating ion flux. Bridging integrator 1 (BIN1) is a T-tubule protein associated with calcium channel trafficking that is downregulated in failing hearts. Here we find that cardiac T tubules normally contain dense protective inner membrane folds that are formed by a cardiac isoform of BIN1. In mice with cardiac Bin1 deletion, T-tubule folding is decreased, which does not change overall cardiomyocyte morphology but leads to free diffusion of local extracellular calcium and potassium ions, prolonging action-potential duration and increasing susceptibility to ventricular arrhythmias. We also found that T-tubule inner folds are rescued by expression of the BIN1 isoform BIN1+13+17, which promotes N-WASP–dependent actin polymerization to stabilize the T-tubule membrane at cardiac Z discs. BIN1+13+17 recruits actin to fold the T-tubule membrane, creating a 'fuzzy space' that protectively restricts ion flux. When the amount of the BIN1+13+17 isoform is decreased, as occurs in acquired cardiomyopathy, T-tubule morphology is altered, and arrhythmia can result.

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IITH Creators:
IITH CreatorsORCiD
Bhargava, Anamikahttp://orcid.org/0000-0002-1048-3427
Item Type: Article
Subjects: Others > Biotechnology
Divisions: Department of Biotechnology
Depositing User: Library Staff
Date Deposited: 20 Aug 2019 05:23
Last Modified: 20 Aug 2019 05:23
URI: http://raiith.iith.ac.in/id/eprint/5959
Publisher URL: http://doi.org/10.1038/nm.3543
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