Isogenic-induced endothelial cells enhance osteogenic differentiation of mesenchymal stem cells on silk fibroin scaffold

Eswaramoorthy1, Sindhuja D and Dhiman, Nandini and Rath, Subha Narayan and et al, . (2019) Isogenic-induced endothelial cells enhance osteogenic differentiation of mesenchymal stem cells on silk fibroin scaffold. regenerative Medicine. ISSN 1746-0751

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Abstract

We investigated the role of induced endothelial cells (iECs) in mesenchymal stem cells (MSCs)/iECs coculture and assessed their osteogenic ability on silk fibroin nanofiber scaffolds.The osteogenic differentiation was assessed by the ALP assay, calcium assay and gene expression studies.The osteogenic differentiation of the iECs co-cultures was found to be higher than the MSCs group and proximal to endothelial cells (ECs) co-cultures. Furthermore, the usage of isogenic iECs for co-culture increased the osteogenic and endothelial gene expression.These findings suggest that iECs mimic endothelial cells when co-cultured with MSCs and that one MSCs source can be used to give rise to both MSCs and iECs. The isogenic MSCs/iECs co-culture provides a new option for bone tissue engineering applications.

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IITH Creators:
IITH CreatorsORCiD
Rath, Subha NarayanUNSPECIFIED
Item Type: Article
Subjects: Biomedical Engineering
Divisions: Department of Biomedical Engineering
Depositing User: Team Library
Date Deposited: 31 Jul 2019 05:34
Last Modified: 31 Jul 2019 05:34
URI: http://raiith.iith.ac.in/id/eprint/5843
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