Label free electrochemical detection of cardiac biomarker troponin T using ZnSnO3 perovskite nanomaterials

Supraja, Patta and Sudarshan, Vadnala and Tripathy, Suryasnata and Agrawal, Amit and Singh, Shiv Govind (2019) Label free electrochemical detection of cardiac biomarker troponin T using ZnSnO3 perovskite nanomaterials. Analytical Methods, 11 (6). pp. 744-751. ISSN 1759-9660

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Abstract

This paper reports label free sub-femtomolar detection of cardiac biomarker troponin T using ZnSnO3 perovskite nanomaterial based electrochemical biosensors. Herein, we have demonstrated two separate synthesis schemes for the perovskite material, one being a hydrothermal synthesis method, and the other being electrochemical deposition. In this work, for the electrochemical detection of troponin T with the hydrothermally synthesized ZnSnO3, we have used a glassy carbon electrode (GCE), on to which the nanomaterials are dropcasted so as to create a heterogeneous working electrode. In a separate scheme, we have used indium tin oxide coated polyethylene terephthalate (ITO/PET) substrates as the working electrodes, on to which a thin film of ZnSnO3 nanomaterial has been deposited electrochemically. Subsequently, the capture antibodies corresponding to the targeted cardiac troponin T are immobilized on to the surface functionalized working electrodes using NHS/EDC chemistry. The electrochemical detection of the target analyte has been performed in the concentration range of 1 fg mL−1 to 1 μg mL−1, using electrochemical impedance spectroscopy. For the GCE and ITO/PET based bioelectrodes, the approximated limits of detection are found to be 0.187 fg mL−1 and 0.571 fg mL−1 respectively, whereas the respective values of sensitivity of the same are 35.25 kΩ (μg mL−1)−1 cm−2 and 8.813 kΩ (μg mL−1)−1 cm−2.

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IITH Creators:
IITH CreatorsORCiD
Singh, Shiv Govindhttp://orcid.org/0000-0001-7319-879X
Item Type: Article
Subjects: Electrical Engineering
Divisions: Department of Electrical Engineering
Depositing User: Team Library
Date Deposited: 26 Feb 2019 06:39
Last Modified: 26 Feb 2019 09:05
URI: http://raiith.iith.ac.in/id/eprint/4840
Publisher URL: http://doi.org/10.1039/C8AY02617C
OA policy: http://www.sherpa.ac.uk/romeo/issn/1759-9660/
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