Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators

Misra, Ashish and Green, Michael R (2016) Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators. In: Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation. Methods in Molecular Biology, 1507 . Springer, New York, pp. 1-12. ISBN 9781493965168

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Abstract

Alternative splicing is a regulated process that leads to inclusion or exclusion of particular exons in a pre-mRNA transcript, resulting in multiple protein isoforms being encoded by a single gene. With more than 90 % of human genes known to undergo alternative splicing, it represents a major source for biological diversity inside cells. Although in vitro splicing assays have revealed insights into the mechanisms regulating individual alternative splicing events, our global understanding of alternative splicing regulation is still evolving. In recent years, genome-wide RNA interference (RNAi) screening has transformed biological research by enabling genome-scale loss-of-function screens in cultured cells and model organisms. In addition to resulting in the identification of new cellular pathways and potential drug targets, these screens have also uncovered many previously unknown mechanisms regulating alternative splicing. Here, we describe a method for the identification of alternative splicing regulators using genome-wide RNAi screening, as well as assays for further validation of the identified candidates. With modifications, this method can also be adapted to study the splicing regulation of pre-mRNAs that contain two or more splice isoforms.

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IITH Creators:
IITH CreatorsORCiD
Misra, AshishUNSPECIFIED
Item Type: Book Section
Uncontrolled Keywords: Alternative splicing, Genome-wide, RNA interference, Flow cytometry, RBFOX2, Pre-mRNA
Subjects: Others > Biotechnology
Divisions: Department of Biotechnology
Depositing User: Team Library
Date Deposited: 16 Nov 2018 07:13
Last Modified: 16 Nov 2018 07:13
URI: http://raiith.iith.ac.in/id/eprint/4549
Publisher URL: http://doi.org/10.1007/978-1-4939-6518-2_1
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