Development of an experimental model of a decellularized kidney scaffold by perfusion mode and analyzing the three-dimensional extracellular matrix architecture by edge detection method

Almelkar, Shahdab I and Bethapudi, Sundeep and Rath, Subha Narayan (2018) Development of an experimental model of a decellularized kidney scaffold by perfusion mode and analyzing the three-dimensional extracellular matrix architecture by edge detection method. Indian Journal of Nephrology, 28 (5). pp. 339-344. ISSN 0971-4065

[img]
Preview
Text
Indian Journal of Nephro_28_5_339-344_2018.pdf - Published Version

Download (1MB) | Preview

Abstract

Renal transplant is treatment of choice for the patients with end stage renal disease. The kidney transplants are expensive and there are risks of immunological and infectious complications. We planned to develop an in vitro decellularized kidney scaffold model using sheep kidney. Kidney decellularization was carried out by perfusing chemical detergents such as sodium dodecyl sulfate (SDS), SDS and trypsin, and SDS and ethylenediaminetetraacetic acid solvent solution. Complete kidney was decellularized in 5 days by perfusing various chemical detergents in time-dependent intervals. Histological finding revealed the complete removal of cellular material in various regions of renal corpuscle, distal convoluted tubules, other cortex and medulla region. Details of interlobular veins and arteries were seen through naked eyes after trypan blue dye injection. We used edge detection technique for developing a three-dimensional (3-D) image (Image J software) for nephrological vasculature constructed of decellularized kidney scaffold specimen. This technique opens a gateway for the whole organ decellularization by perfusion technology and further imaging of its 3-D extracellular matrix texture by edge detection technique software.

[error in script]
IITH Creators:
IITH CreatorsORCiD
Rath, Subha NarayanUNSPECIFIED
Item Type: Article
Uncontrolled Keywords: Histology, Image, Jkidney decellularization, perfusion, trypan blue
Subjects: Biomedical Engineering
Divisions: Department of Biomedical Engineering
Depositing User: Team Library
Date Deposited: 05 Oct 2018 06:43
Last Modified: 05 Oct 2018 06:49
URI: http://raiith.iith.ac.in/id/eprint/4465
Publisher URL: http://doi.org/10.4103/ijn.IJN_14_17
OA policy: http://www.sherpa.ac.uk/romeo/issn/0971-4065/
Related URLs:

Actions (login required)

View Item View Item
Statistics for RAIITH ePrint 4465 Statistics for this ePrint Item