Optimization of solid substrate mixture and process parameters for the production of L-asparaginase and scale-up using tray bioreactor

Doriya, Kruthi and Devarai, Santhosh Kumar (2018) Optimization of solid substrate mixture and process parameters for the production of L-asparaginase and scale-up using tray bioreactor. Biocatalysis and Agricultural Biotechnology, 13. pp. 244-250. ISSN 18788181

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Abstract

L-asparaginase is a key enzyme that degrades asparagine and this aspect of the enzyme has found a dominant role in chemotherapeutic and food processing industry. The aim of the present study is to sequentially optimize L-asparaginase production from newly isolated Aspergillus sp. using agro-industrial residues as solid substrate. In the first stage, through simplex centroid design maximum L-asparaginase activity was observed using a ternary mixture of cotton seed cake (2/3), wheat bran (1/6), and red gram husk (1/6). In the next step, cultivation parameters such as pH, temperature and moisture content were optimized using Box-Behnken design. After 6 days of fermentation using optimized ternary mixture, maximum activity of 12.57 U/mL was obtained at temperature 35 °C, pH-8 and moisture content 70% (v/w). Optimized bounds were further translated to lab-scale tray bioreactor and L-asparaginase activity was found to be 5.41 and 6.67 U/mL in tray bioreactor with 500 g and 1000 g of substrate mixture respectively. L-asparaginase production increased 5 fold through mixture design and 1.3 times increase in enzyme activity was observed with Box-Behnken design. Present work signifies the importance of optimization in the bioprocess industry for complete understanding and evaluation of enzyme production.

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IITH Creators:
IITH CreatorsORCiD
Devarai, Santhosh KumarUNSPECIFIED
Item Type: Article
Uncontrolled Keywords: L-asparaginase, Box-Behnken design, Mixture design, Solid state fermentation, Scale-up
Subjects: Chemical Engineering
Divisions: Department of Chemical Engineering
Depositing User: Team Library
Date Deposited: 16 Jan 2018 09:58
Last Modified: 14 Jan 2019 09:30
URI: http://raiith.iith.ac.in/id/eprint/3720
Publisher URL: http://doi.org/10.1016/j.bcab.2018.01.004
OA policy: http://www.sherpa.ac.uk/romeo/issn/1878-8181/
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