Abnormal Complement Activation and Inflammation in the Pathogenesis of Retinopathy of Prematurity

Rathi, Sonika and Jalali, Subhadra and Patnaik, Satish and Shahulhameed, Shahna and Musada, Ganeswara R and Balakrishnan, Divya and Rani, Padmaja K and Kekunnaya, Ramesh and Chhablani, Preeti Patil and Swain, Sarpras and Giri, Lopamudra and Chakrabarti, Subhabrata and Kaur, Inderjeet (2017) Abnormal Complement Activation and Inflammation in the Pathogenesis of Retinopathy of Prematurity. Frontiers in Immunology, 8. pp. 1-13. ISSN 1664-3224

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Retinopathy of prematurity (ROP) is a neurovascular complication in preterm babies, leading to severe visual impairment, but the underlying mechanisms are yet unclear. The present study aimed at unraveling the molecular mechanisms underlying the pathogenesis of ROP. A comprehensive screening of candidate genes in preterms with ROP (n = 189) and no-ROP (n = 167) was undertaken to identify variants conferring disease susceptibility. Allele and genotype frequencies, linkage disequilibrium and haplotypes were analyzed to identify the ROP-associated variants. Variants in CFH (p = 2.94 x 10(-7)), CFB (p = 1.71 x 10(-5)), FBLN5 (p = 9.2 x 10(-4)), CETP (p = 2.99 x 10(-5)), and CXCR4 (p = 1.32 x 10(-8)) genes exhibited significant associations with ROP. Further, a quantitative assessment of 27 candidate proteins and cytokines in the vitreous and tear samples of babies with severe ROP (n = 30) and congenital cataract (n = 30) was undertaken by multiplex bead arrays and further validated by western blotting and zymography. Significant elevation and activation of MMP9 (p = 0.038), CFH (p = 2.24 x 10(-5)), C3 (p = 0.05), C4 (p = 0.001), IL-1ra (p = 0.0019), vascular endothelial growth factor (VEGF) (p = 0.0027), and G-CSF (p = 0.0099) proteins were observed in the vitreous of ROP babies suggesting an increased inflammation under hypoxic condition. Along with inflammatory markers, activated macrophage/microglia were also detected in the vitreous of ROP babies that secreted complement component C3, VEGF, IL-1ra, and MMP-9 under hypoxic stress in a cell culture model. Increased expression of the inflammatory markers like the IL-1ra (p = 0.014), MMP2 (p = 0.0085), and MMP-9 (p = 0.03) in the tears of babies at different stages of ROP further demonstrated their potential role in disease progression. Based on these findings, we conclude that increased complement activation in the retina/vitreous in turn activated microglia leading to increased inflammation. A quantitative assessment of inflammatory markers in tears could help in early prediction of ROP progression and facilitate effective management of the disease, thereby preventing visual impairment.

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IITH Creators:
IITH CreatorsORCiD
Giri, Lopamudrahttp://orcid.org/0000-0002-2352-7919
Item Type: Article
Uncontrolled Keywords: retina, premature birth, inflammation, genetics, cytokines, abnormal angiogenesis, microglia/macrophage, alternative complement pathway
Subjects: Chemical Engineering
Divisions: Department of Chemical Engineering
Depositing User: Team Library
Date Deposited: 11 Jan 2018 11:50
Last Modified: 11 Jan 2018 11:50
URI: http://raiith.iith.ac.in/id/eprint/3716
Publisher URL: http://doi.org/10.3389/fimmu.2017.01868
OA policy: http://www.sherpa.ac.uk/romeo/issn/1664-3224/
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