Isolation and Screening of Glutaminase & Urease Free Novel Fungal Strains for the Production of L-Asparaginase

Jose, Nimmy (2016) Isolation and Screening of Glutaminase & Urease Free Novel Fungal Strains for the Production of L-Asparaginase. Masters thesis, Indian Institute of Technology Hyderabad.

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Abstract

L - Asparaginase is an amidohydrolase that catalyzes the hydrolysis of amino acid L - aspar a gine in to aspartic acid and ammonia. It is used in the treatment of Acute L ymphoblastic Leukemia (ALL ) and some other malignant lymphoid abnormalities . It is also used in food industry to prevent the formation of a crylamide , a carcinogenic substance in carbohydrate rich fried and baked foods. Naturally L - Asparaginase is present i n plants, animals and microbes but microorganisms such as bacteria, yeast and fungi are generally used for the production of L - Asparaginase as it is difficult to obtain the same from plants and animals. It is found that the L - Asparaginase from bacteria causes side effects in humans including anaphylaxis and serious allergic reactions which can be fatal in some cases . To overcome this, eukaryotic organisms such as fungi can be used for the production of L - Asparaginase . Bu t sometimes the fungi produces L - glutaminase and urease enzymes along with L - Asparaginase which is difficult to remove in the purification stage. In order to prevent this fungal strains which can produce L - Asparaginase fre e of L - glutaminase and urease are isolated from different sources using standard protocols.

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IITH Creators:
IITH CreatorsORCiD
Item Type: Thesis (Masters)
Uncontrolled Keywords: L-asparaginase, Urease, Glutaminase, L-asparagine, TD660
Subjects: Chemical Engineering > Biochemical Engineering
Divisions: Department of Chemical Engineering
Depositing User: Team Library
Date Deposited: 07 Sep 2016 09:19
Last Modified: 07 Sep 2016 09:19
URI: http://raiith.iith.ac.in/id/eprint/2730
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