Quantitative label-free sperm imaging by means of transport of intensity

Popescu, Gabriel and Park, YongKeun and Poola, Praveen Kumar and Pandiyan, V P and Jayaraman, Varshini and John, Renu (2016) Quantitative label-free sperm imaging by means of transport of intensity. In: Quantitative Phase Imaging II, February 13, 2016, Gabriel Popescu; YongKeun Park San Francisco, California, United States.

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Most living cells are optically transparent which makes it difficult to visualize them under bright field microscopy. Use of contrast agents or markers and staining procedures are often followed to observe these cells. However, most of these staining agents are toxic and not applicable for live cell imaging. In the last decade, quantitative phase imaging has become an indispensable tool for morphological characterization of the phase objects without any markers. In this paper, we report noninterferometric quantitative phase imaging of live sperm cells by solving transport of intensity equations with recorded intensity measurements along optical axis on a commercial bright field microscope. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

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IITH Creators:
IITH CreatorsORCiD
John, Renuhttps://orcid.org/0000-0003-3254-2472
Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Live cell imaging ; Microscopes ; Microscopy ; Phase imaging ; Transmittance ; Cells
Subjects: Biomedical Engineering
Divisions: Department of Biomedical Engineering
Depositing User: Team Library
Date Deposited: 18 Aug 2016 05:38
Last Modified: 24 Oct 2018 10:38
URI: http://raiith.iith.ac.in/id/eprint/2656
Publisher URL: https://doi.org/10.1117/12.2211608
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