A surface functionalized nanoporous titania integrated microfluidic biochip

Ali, M A and Srivastava, S and Mondal, K and Chavhan, P M and Agrawal, V V and John, Renu and Sharma, A and Malhotra, B D (2014) A surface functionalized nanoporous titania integrated microfluidic biochip. Nanoscale (6). pp. 13958-13969. ISSN 2040-3364

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Abstract

We present a novel and efficient nanoporous microfluidic biochip consisting of a functionalized chitosan/anatase titanium dioxide nanoparticles (antTiO2-CH) electrode integrated in a polydimethylsiloxane (PDMS) microchannel assembly. The electrode surface can be enzyme functionalized depending on the application. We studied in detail cholesterol sensing using the cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) functionalized chitosan supported mesoporous antTiO2-CH microfluidic electrode. The available functional groups present in the nanoporous antTiO2-CH surface in this microfluidic biochip can play an important role for enzyme functionalization, which has been quantified by the X-ray photoelectron spectroscopic technique. The Brunauer–Emmett–Teller (BET) studies are used to quantify the specific surface area and nanopore size distribution of titania nanoparticles with and without chitosan. Point defects in antTiO2 can increase the heterogeneous electron transfer constant between the electrode and enzyme active sites, resulting in improved electrochemical behaviour of the microfluidic biochip. The impedimetric response of the nanoporous microfluidic biochip (ChEt-ChOx/antTiO2-CH) shows a high sensitivity of 6.77 kΩ (mg dl−1)−1 in the range of 2–500 mg dl−1, a low detection limit of 0.2 mg dl−1, a low Michaelis–Menten constant of 1.3 mg dl−1 and a high selectivity. This impedimetric microsystem has enormous potential for clinical diagnostics applications.

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IITH Creators:
IITH CreatorsORCiD
John, RenuUNSPECIFIED
Item Type: Article
Additional Information: The authors thank Director CSIR-NPL, New Delhi, India for the facilities. We thank Mr Sandeep, AFM Lab, CSIR-NPL, New Delhi for the AFM analysis. Md. A. Ali and S. Srivastava are thankful to CSIR, India for the award of Senior Research Fellowships. We thank Mr Tilak Joshi for providing the facility for ITO patterning using photolithography. Authors are thank ful to Dr Venu Reddy and Prof. CheolGi Kim for the XPS measurements. V. V. A. is thankful to a TSDP-DST & CSIR empowered project for funding, and the DST Unit on Nanoscience at IIT Kanpur is gratefully acknowledged.
Subjects: Biomedical Engineering
Divisions: Department of Biomedical Engineering
Depositing User: Library Staff
Date Deposited: 22 Dec 2015 05:41
Last Modified: 12 Sep 2017 11:05
URI: http://raiith.iith.ac.in/id/eprint/2071
Publisher URL: https://doi.org/10.1039/C4NR03791J
OA policy: http://www.sherpa.ac.uk/romeo/issn/2040-3364/
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