A Framework for Classification of Heterogeneous Calcium Dynamics

Gupta, Rishikesh Kumar (2015) A Framework for Classification of Heterogeneous Calcium Dynamics. Masters thesis, Indian Institute of Technology Hyderabad.

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Abstract

G-protein coupled receptors are the target receptors for designing almost 45% of the drug in current drug market. For drug screening using cell-based assays, fluorescent imaging of GPCR mediated calcium dynamics in single cells can be used to obtain the dose-response profile. However, construction of a dose-response function based on single cell responses is rather challenging as the cells in a population respond heterogeneously to the drug. Here we developed a live cell imaging-based approach to quantify the heterogeneity of the HeLa cell population in response to GPCR mediated drugs. First, we found that activation of CXCR4 by SDF-1α induces calcium responses in Hela cells. We measured the temporal dynamics of cytosolic calcium through time-lapse imaging using confocal microscopy for various drug doses.

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IITH Creators:
IITH CreatorsORCiD
Item Type: Thesis (Masters)
Uncontrolled Keywords: CXCR4, SDF-1α, GPCR, TD427
Subjects: Chemical Engineering > Biochemical Engineering
Divisions: Department of Chemical Engineering
Depositing User: Library Staff
Date Deposited: 03 Aug 2015 06:27
Last Modified: 03 Aug 2015 06:27
URI: http://raiith.iith.ac.in/id/eprint/1754
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