Dielectrophoresis(DEP) and travelling wave dielectrophoresis(TWDEP) based cell manipulation using microfluidics

Karhade, Pallavi (2015) Dielectrophoresis(DEP) and travelling wave dielectrophoresis(TWDEP) based cell manipulation using microfluidics. Masters thesis, Indian Institute of Technology Hyderabad.

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Abstract

Microfluidics is a new trend in medical instrumentation to miniaturize the size of medical devices used in laboratories. Bacterial cell manipulation can be done using “Travelling wave dielectrophoresis(TwDEP)” technique on a Lab on Chip device. In traditional DEP method, polarized cells get either attracted or repelled from electrodes depending on their dielectric properties and size, hence, get separated vertically. In order to collect these separated cells at the outlet hydraulic drag force is required. Whereas in the case of TwDEP cells get vertically separated like normal DEP method and along with it they also get pumped at output, horizontally due to travelling wave. This travelling wave is generated by applying voltage with phase difference at each electrode. Such voltage creates non-uniform electric field with phase shift and hence has force on cells on both the axes. Such devices can be connected to external electric circuits and can be used as bio-sensors.

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IITH Creators:
IITH CreatorsORCiD
Item Type: Thesis (Masters)
Uncontrolled Keywords: DEP, TWDEP, Cell Manipulation, Electrokinetic, TD396
Subjects: Biomedical Engineering
Divisions: Department of Biomedical Engineering
Depositing User: Library Staff
Date Deposited: 13 Jul 2015 05:09
Last Modified: 21 Jun 2017 09:37
URI: http://raiith.iith.ac.in/id/eprint/1696
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