Mutant generation of Mms2 and structural analysis of modeled Uev1a N-terminus docked to Uev1a-Ubc13-CHIP heterotrimer

Mohandas, M L (2015) Mutant generation of Mms2 and structural analysis of modeled Uev1a N-terminus docked to Uev1a-Ubc13-CHIP heterotrimer. Masters thesis, Indian Institute of Technology Hyderabad.

[img] Text
BO13M1005.pdf - Submitted Version
Restricted to Registered users only until 9 July 2018.

Download (3MB) | Request a copy

Abstract

Mms2, a Ubiquitin-conjugating enzyme variant (UEV) protein acts aco-factor for Ubc13 in DNA damage repair. This signaling is mediated by assembly of Lys-63 linked poly- ubiquitin chains by Ubc13 and Mms2 complex. Uev1A, another UEV in conjugation with Ubc13 forms Lys-63 poly-ubiquitin chains which lead to activation of NF-kB pathway. Hence, in the presence of different cofactors, Ubc13 performs distinct biological functions. Mms2 and Uev1A were found to have a sequence similarity of more than 90%. This project aims at generating five mutants of Mms2 by changing the residues to that of Uev1A and studying their interaction with Ubc13

[error in script]
IITH Creators:
IITH CreatorsORCiD
Item Type: Thesis (Masters)
Uncontrolled Keywords: UBC13, Uev1a, MMS2, Docking, TD370
Subjects: Others > Biotechnology
Divisions: Department of Biotechnology
Depositing User: Library Staff
Date Deposited: 08 Jul 2015 10:44
Last Modified: 08 Jul 2015 10:44
URI: http://raiith.iith.ac.in/id/eprint/1666
Publisher URL:
Related URLs:

Actions (login required)

View Item View Item
Statistics for RAIITH ePrint 1666 Statistics for this ePrint Item