Highly efficient bienzyme functionalized nanocomposite-based microfluidics biosensor platform for biomedical application

Md, Azahar Ali and Srivastava, S and Solanki, P R and Reddy, V and Agrawal, V V and Kim, C and John, Renu and Malhotra, B D (2013) Highly efficient bienzyme functionalized nanocomposite-based microfluidics biosensor platform for biomedical application. Scientific Reports, 3 (2661). pp. 1-9. ISSN 2045-2322

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This report describes the fabrication of a novel microfluidics nanobiochip based on a composite comprising of nickel oxide nanoparticles (nNiO) and multiwalled carbon nanotubes (MWCNTs), as well as the chip's use in a biomedical application. This nanocomposite was integrated with polydimethylsiloxane (PDMS) microchannels, which were constructed using the photolithographic technique. A structural and morphological characterization of the fabricated microfluidics chip, which was functionalized with a bienzyme containing cholesterol oxidase (ChOx) and cholesterol esterase (ChEt), was accomplished using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy. The XPS studies revealed that 9.3% of the carboxyl (COOH) groups present in the nNiO-MWCNT composite are used to form amide bonds with the NH 2 groups of the bienzyme. The response studies on this nanobiochip reveal good reproducibility and selectivity, and a high sensitivity of 2.2â.mA/mM/cm 2. This integrated microfluidics biochip provides a promising low-cost platform for the rapid detection of biomolecules using minute samples.

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IITH Creators:
IITH CreatorsORCiD
John, Renuhttps://orcid.org/0000-0003-3254-2472
Item Type: Article
Additional Information: Supplementary information accompanies this paper at http://www.nature.com/ scientificreports
Uncontrolled Keywords: carbon nanotube; cholesterol esterase; cholesterol oxidase; enzyme; metal nanoparticle; nanocomposite; nickel; nickel monoxide
Subjects: Biomedical Engineering
Divisions: Department of Biomedical Engineering
Depositing User: Library Staff
Date Deposited: 10 Mar 2015 05:47
Last Modified: 05 Dec 2017 04:24
URI: http://raiith.iith.ac.in/id/eprint/1387
Publisher URL: https://doi.org/10.1038/srep02661
OA policy: http://www.sherpa.ac.uk/romeo/issn/2045-2322/
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