Lipid–Lipid Interactions in Aminated Reduced Graphene Oxide Interface for Biosensing Application

Azahar Ali, Md and Kamil Reza, K and Srivastava, S and Agrawal, V V and John, Renu and Malhotra, B D (2014) Lipid–Lipid Interactions in Aminated Reduced Graphene Oxide Interface for Biosensing Application. Langmuir, 30 (14). pp. 4192-4201. ISSN 0743-7463

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Abstract

A label-free biosensor based on antiapolipoprotein B 100 functionalized-aminated reduced graphene oxide interface has been fabricated for detection of low density lipoprotein (LDL or lipid) cholesterol. The aminated reduced graphene oxide (NH2-rGO) based electrode surface is covalently functionalized with antiapolipoprotein B 100 (AAB or lipid) using EDC/NHS coupling chemistry. The lipid–lipid interactions at the NH2-rGO electrode surface have been investigated using electrochemical impedance spectroscopic technique. The structural and morphological investigations of NH2-rGO based immunosensor have been accomplished via transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, UV–visible, and electrochemical techniques. The impedimetric response of the proposed immunosensor shows excellent sensitivity (612 Ω mg–1 dL cm–2), a response time of 250 s, and a low detection limit of 5 mg/dL of LDL molecules. The association, dissociation, and equilibrium rate constants for this immunoelectrode are found to be 1.66 M–1 s–1, 0.6 s–1, and 2.77 M–1, respectively. The long-term stability and excellent reproducibility of the proposed immunosensor indicates a suitable platform for detection of LDL or lipid molecules. This immunosensor provides an efficient platform for analysis of the antigen–antibody interactions of lipid molecules.

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IITH Creators:
IITH CreatorsORCiD
John, RenuUNSPECIFIED
Item Type: Article
Uncontrolled Keywords: Fourier transform infrared spectroscopy; Immunosensors; Molecules; Rate constants; Transmission electron microscopy; X ray diffraction Antigen-antibody interaction; Biosensing applications; Electrochemical impedance; Electrochemical techniques; Label-free biosensor; Low density lipoproteins; Reduced graphene oxides; Spectroscopic technique
Subjects: Chemical Engineering > Biochemical Engineering
Divisions: Department of Biotechnology
Depositing User: Users 4 not found.
Date Deposited: 13 Oct 2014 09:22
Last Modified: 12 Sep 2017 11:04
URI: http://raiith.iith.ac.in/id/eprint/121
Publisher URL: https://doi.org/10.1021/la4049852
OA policy: http://www.sherpa.ac.uk/romeo/issn/0743-7463/
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